H2N2V1, Main, Exploration, bibRecord, 002055

Detection of Influenza Viruses in Throat Swab by Using Polymerase Chain Reaction

Identifieur interne : 002055 ( Main/Exploration ); précédent : 002054; suivant : 002056

Detection of Influenza Viruses in Throat Swab by Using Polymerase Chain Reaction

Auteurs : Akira Yamada ; Jiro Imanishi ; Etsuro Nakajima ; Katsuhisa Nakajima [Japon] ; Setsuko Nakajima

Source :

Microbiology and Immunology [ 0385-5600 ] ; 1991-03.

RBID : ISTEX:EEFC42C19CED920A36A8C3A864E719E1A47FBFFB

Descripteurs français

KwdFr :
- ARN viral (analyse), Grippe humaine (diagnostic), Grippe humaine (microbiologie), Gènes viraux (génétique), Humains, Pharynx (microbiologie), Réaction de polymérisation en chaîne, Sensibilité et spécificité, Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus influenza B (génétique), Virus influenza B (isolement et purification), Électrophorèse sur gel d'agar.
MESH :
- analyse : ARN viral.
- diagnostic : Grippe humaine.
- génétique : Gènes viraux, Virus de la grippe A, Virus influenza B.
- isolement et purification : Virus de la grippe A, Virus influenza B.
- microbiologie : Grippe humaine, Pharynx.
- Humains, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Électrophorèse sur gel d'agar.

English descriptors

KwdEn :
- Electrophoresis, Agar Gel, Genes, Viral (genetics), Humans, Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza B virus (genetics), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (microbiology), Pharynx (microbiology), Polymerase Chain Reaction, RNA, Viral (analysis), Sensitivity and Specificity.
MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Influenza, Human.
- genetics : Genes, Viral, Influenza A virus, Influenza B virus.
- isolation & purification : Influenza A virus, Influenza B virus.
- microbiology : Influenza, Human, Pharynx.
- Electrophoresis, Agar Gel, Humans, Polymerase Chain Reaction, Sensitivity and Specificity.

Abstract

An assay protocal based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza virus in throat swab is described. By use of the mixture of HI and H3 primers, it was possible to determine the subtype of the influenza A viruses simultaneously. No visible band was detected after PCR of influenza B or A (H2N2) viruses with a pair of HI or H3 primers. The dilution experiment showed that the influenza viruses, as few as 1.3–6 plaque‐forming units, were sufficient for detecting the HA gene by PCR. All throat swab samples from which influenza viruses had been isolated by conventional method were also positively detected by PCR method.

Url:

https://api.istex.fr/ark:/67375/WNG-5M7K3KR4-L/fulltext.pdf

DOI: 10.1111/j.1348-0421.1991.tb01555.x

Affiliations:

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<front><div type="abstract">An assay protocal based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza virus in throat swab is described. By use of the mixture of HI and H3 primers, it was possible to determine the subtype of the influenza A viruses simultaneously. No visible band was detected after PCR of influenza B or A (H2N2) viruses with a pair of HI or H3 primers. The dilution experiment showed that the influenza viruses, as few as 1.3–6 plaque‐forming units, were sufficient for detecting the HA gene by PCR. All throat swab samples from which influenza viruses had been isolated by conventional method were also positively detected by PCR method.</div>
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Detection of Influenza Viruses in Throat Swab by Using Polymerase Chain Reaction

Detection of Influenza Viruses in Throat Swab by Using Polymerase Chain Reaction

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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